arabidopsis ath1 genome microarray anatomical part: root (Thermo Fisher)
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Arabidopsis Ath1 Genome Microarray Anatomical Part: Root, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Signalling Overlaps between Nitrate and Auxin in Regulation of The Root System Architecture: Insights from the Arabidopsis thaliana"
Article Title: Signalling Overlaps between Nitrate and Auxin in Regulation of The Root System Architecture: Insights from the Arabidopsis thaliana
Journal: International Journal of Molecular Sciences
doi: 10.3390/ijms21082880
Figure Legend Snippet: Signalling mechanism of nitrate mediated auxin modulation induces lateral root growth; ( A ) ( a ) NRT1.1 initially senses, taken up and transport by the NRT1.1 transporter, thereby changing its uptake affinity and modulating phosphorylation to activate signalling pathway. In low nitrate conditions, the phosphorylation switches NRT1.1 into the high-affinity system. This sensing ability of NO 3 − altering NRT1.1 phosphorylation causes calcium (Ca 2+ ) efflux by the activation of phospholipase C (PLC). This results in varying the expression of (TGA1/4*) and nitrate transporter genes ( NRT2.1, NRT2.2, NRT3.1 ) and nitrate assimilation genes ( NIA1 and NiR ). ( b ) T101A/chl1 mutant triggers Ca 2+ -ANR1 signalling from the endosomes under high NO 3 − supply. ( c ) Under adequate NO 3 − supply, AFB3 regulates NAC4 and OBP4 expression, which as a result affect the root renovation. ( d ) Finally, as a result of nitrate assimilation organic nitrogen is produced, this system includes miR167 and miR393, which regulates AFB3 and ARF8 , respectively ( B ) In Auxin response network; Class ARF directly connects to the AuxRE (red arrow). Under low nitrate conditions, AUX/IAAs and TPLs repress transcriptional activation by class-ARFs. The degradation of Aux/IAA released this inhibition by connecting auxin and TIR1/AFB. (See text for further details).*TGA1/4 regulatory factors mediate nitrate response inside Arabidopsis root, and while the link of TGA4 to PLC is not approved. Other transcription factors, for instance, NRG2 , NLP7 , connection to Ca 2+ signalling is currently unknown.
Techniques Used: Activation Assay, Expressing, Mutagenesis, Produced, Inhibition
Figure Legend Snippet: The schematic model represents NO 3 − dependent shoot-root auxin transport and accumulation inside the Arabidopsis root. “A” represents auxin (IAA).
Techniques Used:
Figure Legend Snippet: Molecular players involved in nitrate signalling regulating the primary and lateral roots; Schematic presentation of a regulatory module involved in the nitrate-mediated auxin response in Arabidopsis root. For clarity purposes, all players and their secondary transcription factors (both upstream and downstream) are presented with blue and black colors respectively, which might not be the case in a plant cell. Auxin fluxes and accumulations contribute to primary and lateral root growth are indicated with the black arrow. However, the detailed mechanism and other transcripts are still unknown.
Techniques Used:
Figure Legend Snippet: Affymetrix Arabidopsis ATH1 genome microarray analysis of the root; The schematic model represents the absolute expression level of the different nitrate transcription factors in the root of Arabidopsis , under nitrate (NO 3 − ) and auxin treatment, the red bar represents the gene expression for nitrate treatment, while the green bar represents the expression level for auxin treatments. (See text for further detail).
Techniques Used: Microarray, Expressing